Conventional methods involving H&E are laborious and time consuming. Our technology is instantaneous. We achieve this functionality with several key features: Four channel quantification to reveal structural and metabolic information.Single excitation laser.Tailored spectral window and pulse shaping. Red autofluorescence Imaging channel for Flavin Adenine Dinucleotide (FAD) Provides metabolic information Blue autofluorescence Imaging channel for Nicotinamide Adenine Dinucleotide (NADH)Provides metabolic information Second harmonic generation Provides information on fibrous structues Third harmonic generation Provides information on membrane structures